Download secondary structure of protein10/28/2023 ![]() However, the chemical shifts must first be assigned to particular residues, making the technique considerably slower than the optical methods. NMR chemical shifts may also be used to determine the positions of secondary structure within the primary sequence of a protein. ![]() The frequencies of amide bands are analyzed to determine the distribution of secondary structures in proteins. ![]() Infrared (IR) and Raman spectroscopy require only small volumes of protein solution. Both CD and Raman spectroscopies are particularly useful for measurements over a range of temperatures. Circular dichroism (CD) spectroscopy provides rapid determinations of protein secondary structure with dilute solutions and a way to rapidly assess conformational changes resulting from addition of ligands. The amino acid profile results indicated that Trichilia oilseeds can serve as candidates for addressing protein malnutrition in economically disadvantaged communities of sub-Saharan Africa.Several methods for determination of the secondary structure of proteins by spectroscopic measurements are reviewed. The results showed that the essential amino acid levels of the two Trichilia seed proteins were above the recommended Food and Agricultural Organisation standard for adults, with essential-to-total (E/T) amino acid ratios (T. dregeana 52.8% T. emetica 53.4%) and chemical scores of 100% for both species, which were not significantly different (P > 0.05) from those of soy proteins (53.0% and 100%, respectively). This study determined and compared the amino acid profile, functional properties, and secondary structure of the proteins from Trichilia emetica and Trichilia dregeana oilseeds. This problem could be resolved through the nutritional use of locally available underutilized tree seeds such as those from Trichilia species. The high prevalence of protein energy malnutrition (PEM) is a major concern in Africa. ![]()
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